A HUN-REN BRC & HCEMM Research and Resource Facility

Group leader

Zsuzsanna Darula

We accept samples for qualitative and relative quantitative protein analyses including


Sample preparation

  • Affinity isolation of protein complexes by co-immunoprecipitation of (tagged) bait proteins

  • Protein digestion using different proteases (trypsin, chymotrypsin, GluC, LysC)

  • High-pH reversed phase peptide separation for offline fractionation of complex samples

  • Isobaric labeling of peptides for relative quantification (TMT reagent)

  • Peptide purification using C18 tips or cartridges

  • Isolation of phosphopeptides using IMAC (immobilized metal affinity chromatography)


Mass Spectrometry and data analysis

  • LC-MS/MS analysis of peptides using HCD, CID and/or ETD fragmentation

  • Protein identification with database search (Protein Prospector, Byonic, Mascot)

  • Relative quantification of proteins using label-free or isobaric labeling methods (Proteome Discoverer, Skyline)



Mass Spectrometry

Orbitrap Fusion Lumos Tribrid (Thermo)

Orbitrap Elite (Thermo)

LCQ Fleet (Thermo)


Liquid Chromatography

ACQUITY UPLC M-Class system (Waters) – directly interfaced to the OT Lumos for online LC-MS/MS

nanoAcquity UPLC system (Waters) – directly interfaced to the OT Elite for online LC-MS/MS

Jasco and Eldex HPLC systems for offline sample fractionation

Please contact the research group prior to starting sample preparation in order to determine the best approach to analyze your samples.