Affinity isolation of protein complexes by co-immunoprecipitation of (tagged) bait proteins
Protein digestion using different proteases (trypsin, chymotrypsin, GluC, LysC)
High-pH reversed phase peptide separation for offline fractionation of complex samples
Isobaric labeling of peptides for relative quantification (TMT reagent)
Peptide purification using C18 tips or cartridges
Isolation of phosphopeptides using IMAC (immobilized metal affinity chromatography)
LC-MS/MS analysis of peptides using HCD, CID and/or ETD fragmentation
Protein identification with database search (Protein Prospector, Byonic, Mascot)
Relative quantification of proteins using label-free or isobaric labeling methods (Proteome Discoverer, Skyline)
Orbitrap Fusion Lumos Tribrid (Thermo)
Orbitrap Elite (Thermo)
LCQ Fleet (Thermo)
ACQUITY UPLC M-Class system (Waters) – directly interfaced to the OT Lumos for online LC-MS/MS
nanoAcquity UPLC system (Waters) – directly interfaced to the OT Elite for online LC-MS/MS
Jasco and Eldex HPLC systems for offline sample fractionation