SERVICES
We accept samples for qualitative and relative quantitative protein analyses including
Sample preparation
Affinity isolation of protein complexes by co-immunoprecipitation of (tagged) bait proteins
Protein digestion using different proteases (trypsin, chymotrypsin, GluC, LysC)
High-pH reversed phase peptide separation for offline fractionation of complex samples
Isobaric labeling of peptides for relative quantification (TMT reagent)
Peptide purification using C18 tips or cartridges
Isolation of phosphopeptides using IMAC (immobilized metal affinity chromatography)
Mass Spectrometry and data analysis
LC-MS/MS analysis of peptides using HCD, CID and/or ETD fragmentation
Protein identification with database search (Protein Prospector, Byonic, Mascot)
Relative quantification of proteins using label-free or isobaric labeling methods (Proteome Discoverer, Skyline)
INSTRUMENTATION
Mass Spectrometry
Orbitrap Fusion Lumos Tribrid (Thermo)
Orbitrap Elite (Thermo)
LCQ Fleet (Thermo)
Liquid Chromatography
ACQUITY UPLC M-Class system (Waters) – directly interfaced to the OT Lumos for online LC-MS/MS
nanoAcquity UPLC system (Waters) – directly interfaced to the OT Elite for online LC-MS/MS
Jasco and Eldex HPLC systems for offline sample fractionation
SAMPLE SUBMISSION
Please contact the research group prior to starting sample preparation in order to determine the best approach to analyze your samples. For some general guidelines and practical hints for sample preparation please click here.