Laboratory of Functional Genomics

Genomics Unit

Until the second half of the twentieth century, the study of the function and regulation of genes was based on a step-by-step analysis of individual genes. As genomes of more and more organisms become fully or partially known, a number of new technologies have been developed that allow systematic and/or individual analysis of gene function. The Laboratory of Functional Genomics operating at the Szeged Biological Center since 2000, offers the possibility of individual or systematic analysis of gene expression, quantitative analysis of nucleic acids (RNA, DNA, miRNA, blood-derived circulating cell-free cfRNA) and DNA methylation on several platforms. The laboratory conducts research activities and operates partly on a service and partly on a collaborative basis. Our main developments: amplification of nucleic acids from small tissue samples or few cells, single cell RNA sequencing methods, digital nucleic acid detections, bioinformatic analysis of gene expression studies.

Publication of the last 5 years in genomics

1. Köhler, Zoltán Márton ; Trencsényi, György ; Juhász, László ; Zvara, Ágnes ; Szabó, P Judit ; Dux, László ; Puskás, G László ; Rovó, László ; Keller-Pintér, Anikó, Tilorone increases glucose uptake in vivo and in skeletal muscle cells by enhancing Akt2/AS160 signaling and glucose transporter levels, JOURNAL OF CELLULAR PHYSIOLOGY 238 : 5 pp. 1080-1094. , 15 p. (2023)

2. Shukla, R.D. ; Zvara, Á. ; Avramucz, Á. ; Biketova, A.Yu. ; Nyerges, A. ; Puskás, L.G. ; Fehér, T., inPOSE: A Flexible Toolbox for Chromosomal Cloning and Amplification of Bacterial Transgenes, MICROORGANISMS 10 : 2 Paper: 236 (2022

3. Tóth, Melinda E. ✉ ; Sárközy, Márta* ✉ ; Szűcs, Gergő ; Dukay, Brigitta ; Hajdu, Petra ; Zvara, Ágnes ; Puskás, László G. ; Szebeni, Gábor J. ; Ruppert, Zsófia ; Csonka, Csaba et al. Exercise training worsens cardiac performance in males but does not change ejection fraction and improves hypertrophy in females in a mouse model of metabolic syndrome BIOLOGY OF SEX DIFFERENCES 13 : 1 Paper: 5 , 20 p. (2022)

4. Váczi, S. ; Barna, L. ; Harazin, A. ; Mészáros, M. ; Porkoláb, G. ; Zvara, Á. ; Ónody, R. ; Földesi, I. ; Veszelka, S. ; Penke, B. et al. S1R agonist modulates rat platelet eicosanoid synthesis and aggregation, PLATELETS 33 : 5 pp. 709-718. , 10 p. (2022)

5. Balogh A, Reiniger L, Hetey S, Kiraly P, Toth E, Karaszi K, Juhasz K, Gelencser Z, Zvara A, Szilagyi A, Puskas LG, Matko J, Papp Z, Kovalszky I, Juhasz C, Than NG. Decreased Expression of ZNF554 in Gliomas is Associated with the Activation of Tumor Pathways and Shorter Patient Survival. Int J Mol Sci. 2020 Aug 11;21(16):5762. doi: 10.3390/ijms21165762. PMID: 32796700; PMCID: PMC7461028.

6. Barna L, Walter FR, Harazin A, Bocsik A, Kincses A, Tubak V, Jósvay K, Zvara Á, Campos-Bedolla P, Deli MA. Simvastatin, edaravone and dexamethasone protect against kainate-induced brain endothelial cell damage. Fluids Barriers CNS. 2020 Feb 10;17(1):5. doi: 10.1186/s12987-019-0166-1. PMID: 32036791; PMCID: PMC7008534.

7. Gyukity-Sebestyén E, Harmati M, Dobra G, Németh IB, Mihály J, Zvara Á, Hunyadi-Gulyás É, Katona R, Nagy I, Horváth P, Bálind Á, Szkalisity Á, Kovács M, Pankotai T, Borsos B, Erdélyi M, Szegletes Z, Veréb ZJ, Buzás EI, Kemény L, Bíró T, Buzás K. Melanoma-Derived Exosomes Induce PD-1 Overexpression and Tumor Progression via Mesenchymal Stem Cell Oncogenic Reprogramming. Front Immunol. 2019 Oct 18;10:2459. doi: 10.3389/fimmu.2019.02459. PMID: 31681332; PMCID: PMC6813737.

8. Bencsik P, Kiss K, Ágg B, Baán JA, Ágoston G, Varga A, Gömöri K, Mendler L, Faragó N, Zvara Á, Sántha P, Puskás LG, Jancsó G, Ferdinandy P. Sensory Neuropathy Affects Cardiac miRNA Expression Network Targeting IGF-1, SLC2a-12, EIF-4e, and ULK-2 mRNAs. Int J Mol Sci. 2019 Feb 25;20(4):991. doi: 10.3390/ijms20040991. PMID: 30823517; PMCID: PMC6412859.

9. Fenteany, Gabriel* ; Gaur, Paras* ; Hegedűs, Lili ; Dudás, Kata ; Kiss, Ernő ; Wéber,Edit ; Hackler, László ; Martinek, Tamás ; Puskás, László G. ; Haracska, Lajos Multilevel structure–activity pro􀅣ling reveals multiple green tea compound familiesthat each modulate ubiquitin-activating enzyme and ubiquitination by a distinctmechanism, SCIENTIFIC REPORTS 9 : 1 Paper: 12801 , 16 p. (2019)

10. Sárközy M, Gáspár R, Zvara Á, Kiscsatári L, Varga Z, Kővári B, Kovács MG, Szűcs G, Fábián G, Diószegi P, Cserni G, Puskás LG, Thum T, Kahán Z, Csont T, Bátkai S. Selective Heart Irradiation Induces Cardiac Overexpression of the Pro- hypertrophic miR-212. Front Oncol. 2019 Jul 16;9:598. doi: 10.3389/fonc.2019.00598. PMID: 31380269; PMCID: PMC6646706.

11. Sárközy M, Gáspár R, Zvara Á, Siska A, Kővári B, Szűcs G, Márványkövi F, Kovács MG, Diószegi P, Bodai L, Zsindely N, Pipicz M, Gömöri K, Kiss K, Bencsik P, Cserni G, Puskás LG, Földesi I, Thum T, Bátkai S, Csont T. Chronic kidney disease induces left ventricular overexpression of the pro-hypertrophic microRNA-212. Sci Rep. 2019 Feb 4;9(1):1302. doi: 10.1038/s41598-018-37690-5. PMID: 30718600; PMCID: PMC6362219.

12. Csajbók ÉA, Kocsis ÁK, Faragó N, Furdan S, Kovács B, Lovas S, Molnár G, Likó I, Zvara Á, Puskás LG, Patócs A, Tamás G. Expression of GLP-1 receptors in insulin-containing interneurons of rat cerebral cortex. Diabetologia. 2019 Apr;62(4):717-725. doi: 10.1007/s00125-018-4803-z. Epub 2019 Jan 12. PMID: 30637442.

Cytometry unit

The cooperation of highly specialized cell types maintains the homeostasis of multicellular organisms. The disturbance of that harmony contributes to the development of several diseases. Most of the cellular functions are executed by proteins so it is essential to investigate biological processes at the protein level. One of the routinely used methods to study cellular proteins is flow cytometry which detects cell surface or intracellular proteins by fluorescently labeled antibodies at single cell resolution. Currently, we use BC Cytoflex S (4 lasers), one BD FACSCalibur (2 lasers) analyzators and one BD Jazz (2 lasers) sorter. Overlap among the fluorescence spectra of different dyes limits the possibility of high multiplexicity in one single tube. To overcome the limitations of flow cytometry, antibodies are labeled by stable heavy metal isotopes (min 96% monoisotopic) in mass cytometry. Mass cytometer detects the distinct atomic mass of heavy metal isotopes which offers the possibility to acquire up to 42 markers in one sample. Mass cytometry is unique technology in our laboratory within Hungary. The characterization of cellular heterogeneity is achieved at the protein level with single cell resolution from homogenous cell suspensions of different biological samples (blood, solid tumors, different tissues) followed by antibody staining for mass cytometry. High content data analysis (Cytobank, Astrolabe, Maxpar Pathsetter) is also integrated in our portfolio.

We focus on the disturbances of the activation and regulation of immune system; therefore we study human patient-derived blood or tissue samples at single cell resolution. The high content immunophenotyping is performed on PBMCs or different tissues such as primary adenocarcinoma.

The comparative bioinformatic analysis of biological specimens (patients vs healthy controls) reveals differences in the expression pattern of proteins of interest at single cell resolution. To complement the investigation of PBMCs we quantify the cytokine/chemokine content of the corresponding liquid biopsies by Luminex (MagPix) or Legendplex (Biolegend) technologies. We describe expression patterns of proteins associated with a given pathology such as rheumatoid arthritis, adenocarcinoma, COPD to find diagnostic, prognostic markers or novel therapeutic targets. We develop in vitro/ex vivo functional immune assays (i) to screen drug candidate molecules, (ii) to better understand the pathomechanism and reveal signal transduction pathways behind the given pathologies.

The laboratory is run on service and collaborative basis. We have built wide national and international connections to research groups and biotech companies.

Main research areas

• Multiparametric immunophenotyping of systemic autoimmune disorders (RA, SLE, SSC)

• Single cell mass cytometric characterization of the inflammatory complications in type II diabetes mellitus

• Investigation of plasma proteins and single cell PBMCs in stable vs exacerbating COPD

• High multiplex investigation of the intratumor heterogeneity in human primary adenocarcinoma

• Studying the innate immunity in Drosophila melanogaster mutant hemocytes by single cell mass cytometry

• Analysis of the effect of drug candidate molecules using single cell mass cytometry

Publications from the last 5 years in cytomtery

1. Nikolett Gémes, József Á Balog, Patrícia Neuperger, Erzsébet Schlegl, Imre Barta, János Fillinger, Balazs Antus, Ágnes Zvara, Zoltán Hegedűs, Zsolt Czimmerer, Máté Manczinger, Gergő Balogh, József Tóvári, László G Puskás and Gabor Janos Szebeni, Single cell immunophenotyping revealed the association of CD4+ central-and CD4+ effector memory T-cells linking exacerbating chronic obstructive pulmonary disease and NSCLC Frontiers in Immunology 2023, accepted for publication, IF (2022): 7.30 (Scimago Immunology Q1)

2. Patrícia Neuperger, Klára Szalontai, Nikolett Gémes, József Á Balog, László Tiszlavicz, József Furák, György Lázár, Laszlo G Puskas and Gábor J. Szebeni, Single-Cell Mass Cytometric Analysis of Peripheral Immunity and Multiplex Plasma Marker Profiling of Non-Small Cell Lung Cancer Patients Receiving PD-1 Targeting Immune Checkpoint Inhibitors in Comparison with Platinum-Based Chemotherapy, Frontiers in Immunology 2023 Oct 13:14:1243233. eCollection 2023 IF (2022): 7.30 (Scimago Immunology Q1)

3. Seyyed Ashkan Senobar Tahaei, Ágnes Kulmány, Renáta Minorics, Anita Kiss, Zoltán Szabó, Péter Germán, Gábor J. Szebeni, Nikolett Gémes, Erzsébet Mernyák, István Zupkó, Antiproliferative and antimetastatic properties of 16-azidomethyl substituted 3-O-benzyl estrone analogs Int. J. Mol. Sci. 2023 24(18), 13749; IF (2022): 5.60 (Scimago Medicine Q1)

4. Tamas Kiss; Yaqub Mir; Gergely Stefancsik; Ganbat Gantulga; Aruzhan Askarova; Eva Monostori; Karolina Dulka; Gabor Szebeni; Ádám Nyúl-Tóth; Anna Csiszár, Ádám Légrádi, Galectin-1 as a marker for microglia activation in the aging brain, Brain Research 2023 Aug 7;1818:148517. IF (2022): 2.9 (Scimago Neurology Q2)

5. Gábor J. Szebeni, Róbert Alföldi, Lajos I. Nagy, Patrícia Neuperger, Nikolett Gémes, József A. Balog, László Tiszlavicz, László G. Puskás, Introduction of an ultraviolet C irradiated 4T1 murine breast cancer whole cell vaccine model, Vaccines 2023, 11(7), 1254; IF (2022): 7.80 (Scimago Immunology Q1)

6. Enikő Szabó, Szabolcs Modok2, Benedek Rónaszéki, Anna Faragó, Nikolett Gémes, Lajos I. Nagy, László Hackler Jr., Katalin Farkas, Patrícia Neuperger, József Á. Balog, Attila Balog, László G. Puskás and Gabor J. Szebeni, Comparison of humoral and cellular immune responses in hematologic diseases following completed vaccination protocol with BBIBP-CorV, or AZD1222, or BNT162b2 vaccines against SARS-CoV-2, Frontiers in Medicine, 2023 Jul 17;10:1176168; IF (2022): 3.90 (Scimago Medicine Q1)

7. Viktória Nagy, Raji Mounir, Gábor J. Szebeni, Zsolt Szakonyi, Nikolett Gémes, Renáta Minorics, Péter Germán, Zupkó István, Investigation of anticancer properties of monoterpene-aminopyrimidine hybrids on A2780 ovarian cancer cells, Int. J. Mol. Sci. 2023, 24(13), 10581 IF (2022): 5.60 (Scimago Medicine Q1)

8. Apolka Domokos, Zsofia Varga, Karoly Jambrovics, Noemi Cabllero Sanchez, Eniko Szabo, Gergely Nagy, Beata Scholtz, Laszlo Halasz, Eszter Varadi, Krisztian Bene, Anett Mazlo, Attila Bacsi, Viktoria Jeney, Gabor Szebeni, Laszlo Nagy, Zsolt Czimmerer, The transcriptional control of the VEGFA-VEGFR1 (FLT1) axis in alternatively polarized murine and human macrophages, Frontiers in Immunology 2023 May 4;14:1168635. Research Topic Transcriptional Regulation of Macrophage Function, IF (2022): 7.30 (Scimago Immunology Q1)

9. Hazhmat Ali, Péter Traj, Gábor J. Szebeni, Nikolett Gémes, Vivien Resch, Gábor Paragi, Erzsébet Mernyák, Renáta Minorics*, Istvan Zupko*, Investigation of antineoplastic effects of 13α-estrone sulfamate analogs against the HPV-16 positive human invasive cervical carcinoma cell line SiHa, Int. J. Mol. Sci. 2023Apr 1;24(7):6625. IF (2022): 5.60 (Scimago Medicine Q1)

10. Nikolett Gémes, Zsófia Makra, Patrícia Neuperger, Enikő Szabó, József Á. Balog, Lili Borbála Flink, Beáta Kari, László Hackler Jr., László. G. Puskás, Iván Kanizsai*, and Gábor J. Szebeni* A Cytotoxic Survey On 2-Amino-1H-Imidazol Based Synthetic Ma-rine Sponge Alkaloid Analogues, Drug Development Research, 2022 Dec;83(8):1906-1922. IF (2022): 3.80 (Scimago Drug Discovery Q3)

11. Dániel Honfi, Nikolett Gémes, Enikő Szabó, Patrícia Neuperger, József Á. Balog, Lajos I. Nagy, Gergely Toldi, László G. Puskás, Gábor J. Szebeni* and Attila Balog*, Comparison of homologous and heterologous booster SARS-CoV-2 vaccination in autoimmune rheumatic and musculoskeletal patients, Special Issue: “Immunophenotyping in Autoimmune Diseases and Cancer 2.0” Int. J. Mol. Sci. 2022, 23(19), 11411, IF (2022): 5.60 (Scimago Medicine Q1)

12. Kupai, K.; Várkonyi, T.; Török, S.; Gáti, V.; Czimmerer, Z.; Puskás, L.G.; Szebeni, G.J.* Recent Progress in the Diagnosis and Management of Type 2 Diabetes Mellitus in the Era of COVID-19 and Single Cell Multi-Omics Technologies. Life 2022, 12, 1205, IF (2022): 3.20 (Scimago Biochemistry Q2)

13. Gábor J. Szebeni*, Nikolett Gémes, Dániel Honfi, Enikő Szabó, Patrícia Neuperger, József Á. Balog, Lajos I. Nagy, Zoltan Szekanecz, Laszlo G Puskas, Gergely Toldi, Attila Balog*, Humoral and Cellular Immunogenicity and Safety of Five Different SARS-CoV-2 Vaccines in Patients With Autoimmune Rheumatic and Musculoskeletal Diseases in Remission or With Low Disease Activity and in Healthy Controls: A Single Center Study. Frontiers in Immunology, 2022 Mar 31;13:846248, IF (2022): 7.30 (Scimago Immunology Q1)

14. Melinda E. Tóth; Marta Sarkozy; Gergő Szűcs; Brigitta Dukay; Petra Hajdu; Ágnes Zvara; László G. Puskás; Gábor J. Szebeni; Zsófia Ruppert; Csaba Csonka; Ferenc Kovács; András Kriston; Péter Horváth; Bence Kővári; Gábor Cserni; Tamás Csont; Miklós Sántha Exercise training worsens cardiac performance in males but does not change ejection fraction and improves hypertrophy in females in a mouse model of metabolic syndrome, Biology of Sex Differences, 2022 Jan 31;13(1):5, IF (2022): 7.90 (Scimago Endocrinology Q1)

15. Neuperger, P.; Balog, J.Á.; Tiszlavicz, L.; Furák, J.; Gémes, N.; Kotogány, E.; Szalontai, K.; Puskás, L.G.*; Szebeni, G.J.* Analysis of the Single-Cell Heterogeneity of Adenocarcinoma Cell Lines and the Investigation of Intratumor Heterogeneity Reveals the Expression of Transmembrane Protein 45A (TMEM45A) in Lung Adenocarcinoma Cancer Patients. Cancers 2022, 14, 144. IF (2022): 5.20 (Scimago Oncology: Q1)

16. Szilvia Brunner, Dániel Varga, Renáta Bozó, Róbert Polanek, Tünde Tőkés, Emília Rita Szabó, Réka Molnár, Nikolett Gémes, Gábor Szebeni, László G Puskás, Miklós Erdélyi, Katalin Hideghéty Analysis of ionizing radiation induced DNA damage by superresolution dSTORM microscopy, Pathology & Oncology Research, 2021 Nov 8;27:1609971. IF (2021): 2.874 (Scimago Medicine Q2)

17. Renáta Szabó, Denise Börzsei, Alexandra Hoffmann, Zelma Nadin Lesi, Rudolf Gesztelyi, Béla Juhász, Gábor J. Szebeni, Jasmin Osman, Judith Sebestyén, Arnold Nagy, Sándor Szegedi, Csaba Varga, and Anikó Pósa Lifestyle-induced redox-sensitive alterations: Cross-talk among the RAAS, antioxidant/inflammatory status, and hypertension, Oxidative Medicine and Cellular Longevity, Volume 2021, Article ID 3080863, IF (2021): 7.31 (Scimago Medicine Q1)

18. Ágnes E. Kulmány, Éva Frank, András Szekeres, Gábor J. Szebeni and István Zupkó, Biological Evaluation of Antiproliferative and Anti-Invasive Properties of an Androstadiene Derivative on Human Cervical Cancer Cell Lines, Journal of Steroid Biochemistry and Molecular Biology, IF (2021): 5.011 (Scimago Clinical Biochemistry Q1)

19. Klára Szalontai, Nikolett Gémes, József Furák, Tünde Varga, Patrícia Neuperger, József Á. Balog, László G. Puskás and Gábor J. Szebeni*, Chronic Obstructive Pulmonary Disease: Epidemiology, Biomarkers and Paving the Way to Lung Cancer, Journal of Clinical Medicine 2021, Special issue: Living with Chronic Obstructive Pulmonary Disease, 2021 Jul; 10(13): 2889. IF (2021): 4.964 (Scimago Medicine Q1)

20. Neuperger Patrícia, Puskás László G., Szebeni Gábor János*, Single cell mass cytometric comparison of human A549 lung and MDA-MD-231 breast adenocarcinoma cellular models, Hungarian Oncology 2021, 2021 Jun 3;65(2):129-138. Scimago Oncology Q4

21. Ágnes E. Kulmány, Éva Frank, Dóra Kovács, Kerstin Kirisits, Georg Krupitza, Patrícia Neuperger, Róbert Alföldi, László G. Puskás, Gábor J. Szebeni and István Zupkó Antiproliferative and Antimetastatic Characterization of an Exo-heterocyclic Androstane Analog Against Human Breast Cancer Cell Lines Biomedicine & Pharmacotherapy 2021 May 19;140:111728, IF (2021): 7.419 (Scimago Medicine Q1)

22. Jozsef A. Balog, Agnes Kemeny, Laszlo G. Puskas, Szilard Burcsar, Attila Balog, * and Gabor J. Szebeni* Investigation of newly diagnosed drug-naive patients with systemic autoimmune diseases revealed the cleaved peptide tyrosine tyrosine (PYY 3-36) as a specific plasma biomarker of rheumatoid arthritis, Mediators of Inflammation, Jun 17;2021:5523582. Special issue: Inflammation and Regional Immune Balance in Autoimmune Disorders IF (2021): 4.529 (Scimago Immunology Q2)

23. Melinda E. Tóth *, Brigitta Dukay, Mária Péter, Gábor Balogh, Gergő Szűcs, Ágnes Zvara, Gábor J. Szebeni, Petra Hajdu, Márta  Sárközy, László G. Puskás, Zsolt Török, Tamás Csont, László Vígh,  Miklós Sántha, Male and female animals respond differently to high-fat diet and regular exercise training in a mouse model of hyperlipidemia, Int. J. Mol. Sci. 2021, 22(8), 4198, IF (2021): 6.208 (Scimago Medicine Q1)

24. József Á. Balog, Viktor Honti, Éva Kurucz, Beáta Kari, László G. Puskás, István Andó*, Gábor J. Szebeni*, Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry, Genomics Proteomics & Bioinformatics, 2021 Mar 10;S1672-0229(21)00055-3, IF (2021): 6.409 (Scimago Genetics Q1)

25. Kotogány, E.; Balog, J.Á.; Nagy, L.I.; Alföldi, R.; Bertagnolo, V.; Brugnoli, F.; Demjén, A.; Kovács, A.K.; Batár, P.; Mezei, G.; Szabó, R.; Kanizsai, I.; Varga, C.; Puskás, L.G.; Szebeni, G.J. Imidazo[1,2-b]pyrazole-7-Carboxamide Derivative Induces Differentiation-Coupled Apoptosis of Immature Myeloid Cells Such as Acute Myeloid Leukemia and Myeloid-Derived Suppressor Cells. Int. J. Mol. Sci. 2020, 21, 5135.

26. Roberta Fajka-Boja, Gábor J Szebeni, Éva Hunyadi-Gulyás, László G Puskás, Robert Katona, Polyploid adipose stem cells shift the balance of IGF1/IGFBP2 to promote the growth of breast cancer, Frontiers in Oncology, section Molecular and Cellular Oncology, 2020;10:157

27. József Á. Balog,László Hackler Jr.,Anita K. Kovács,Patrícia Neuperger,Róbert Alföldi,Lajos I. Nagy,László G. Puskás and Gábor J. Szebeni, Single Cell Mass Cytometry Revealed the Immunomodulatory Effect of Cisplatin Via Downregulation of Splenic CD44+, IL-17A+ MDSCs and Promotion of Circulating IFN-γ+ Myeloid Cells in the 4T1 Metastatic Breast Cancer Model Int. J. Mol. Sci. 2020, 21(1), 170; 

28. Alföldi R, Balog JÁ, Faragó N, Halmai M, Kotogány E, Neuperger P, Nagy LI, Fehér LZ, Szebeni GJ, Puskás LG. Single Cell Mass Cytometry of Non-Small Cell Lung Cancer Cells Reveals Complexity of In vivo And Three-Dimensional Models over the Petri-dish. Cells. 2019 Sep 16;8(9). pii: E1093. doi: 10.3390/cells8091093. Special Issue: “Single cell Analysis”

29. Gábor J. Szebeni, Lajos I. Nagy, Anikó Berkó, Alexandra Hoffmann, Liliána Z. Fehér, Mária Bagyánszki , Beáta Kari, József A. Balog, László Hackler Jr. ,Iván Kanizsai, Anikó Pósa, Csaba Varga and László G. Puskás The Anti-Inflammatory Role of Mannich Curcuminoids; Special Focus on Colitis, Molecules, Special Issue: “Anti-Inflammatory Activity of Natural Products” 2019, 24(8), 1546

Grants of the group from the last 8 years

GINOP-2.3.2-15-2016-00001

GINOP-2.3.2-15-2016-00028

GINOP-2.3.2-15-2016-00030

GINOP-2.2.1-15-2017-00054

2018-1.3.1-VKE-2018-00018

2018-1.3.1-VKE-2018-00024

UNKP-19-4-SZTE-36

UNKP-18-4-SZTE-73

BO/00139/17/8

KDP-2020/1000464

2020-1.1.6-JÖVŐ-2021-00003

ÚNKP-22-5 -SZTE-535

KDP-2021/C1764415

BO00582/22/8,

FK142877

ÚNKP-23-5 -SZTE-694