Group leader: Zoltán Lipinszki

Email: lipinszki.zoltan[at]brc.hu

Group website:

Group members

Name

Title

 

 

Cell cycle unit:

 

 

 

Zoltán LIPINSZKI

senior research associate, principal investigator

 

publications

CV

Andor UDVARDY

professor emeritus

publications

CV

 

Edit TEMESVÁRINÉ Ábrahám

 

research associate

publications

CV

 

Lilla FÁBRI-ÖRDÖGH

 

research associate

publications

CV

 

Zsuzsánna RÉTHI-NAGY

 

PhD student

publications

CV

 

Kinga DÁN

undergraduate student

publications

CV

 

András SÁRKÖZY

 

scientific adminsitrator

publications

CV

 

Katalin UDVARDY

 

voluntary laboratory assistant

 

 

Gáborné BAKACSI

technician

 

 

 

Name

Transcription unit:

Title

 

 

Imre Miklós

BOROS

 

scientific adviser, principal investigator

 

publications

CV

 

Andrea ÁBRAHÁM

 

PhD student

publications

CV

 

Gyuláné ÖKRÖS

laboratory assistant

 

 

Research                                  

PROJECT 1. The role of protein phosphatases in cell cycle regulation

(supervisor: Zoltan Lipinszki)

The misregulation of cell division during the animal cell cycle results in serious developmental defects or cell proliferative diseases, such as cancer. Fundamental to screening for or correcting the resultant abnormalities in humans is defining their underlying molecular etiology. It is well established that kinase-mediate protein phosphorylation can serve as a molecular switch to fine-tune the activities of different molecular complexes during cell division. Key to the success of this regulatory mechanism is its reversibility; an antagonistic enzymatic activity driven by protein phosphatases. Despite this recognition, relatively little is known about how protein phosphatases govern cell division.

Our group is interested in the characterization and functional analysis of PP2A-like Ser/Thr phosphoprotein phosphatases. PP2A phosphatases are comprised of one evolutionarily conserved catalytic subunit, a structural subunit and a variable number of regulatory 3 subunits, or R3s. The R3 subunit is responsible for the subcellular localization of the holoenzyme as well as the selective binding to interacting partners or particular substrates of the phosphatase. Towards defining the complex interplay and regulation of PP2A-like phosphatases we utilise the Drosophila melanogaster model system. The fruit fly recapitulates the phosphatase system observed in humans but with the ability to integrate biochemical, cell biological, proteomic and genetic experimental approaches.

 

PROJECT 2. The role of alternative histones and histone modifications

(supervisor: Imre Boros)

The chromatin structure plays important roles in gene expression regulation. Consequently the roles of histone proteins which together with DNA constitute chromatin and different types of histone modifications are central topics in studies concerning gene functions. By extension of our knowledge on histone functions we can expect to reveal causes of gene expression alterations leading to various diseases.

In collaboration with researchers at Szeged University Department of Biochemistry and Molecular biology we study the role of specific histones using modern molecular biology and genetic approaches. Our goal is describe the roles of some of the alternative histones used under specific developmental or physiological conditions. One direction of ongoing research is to find explanation why is a specific linker histone required at the start of embryogenesis in higher eukaryotes. Based on recent result we obtained with the use of Drosophila model we propose that the embryonic linker histone is required to permit rapid chromatin reorganisations needed for quick replication cycles and genome activation at the beginning of embryogenesis. In another ongoing project we study the role of a histone 4 protein expressed from a non-canonical histone gene. We use state of the art genomic approaches to reveal whether this structurally conserved H4 protein is involved in establishment of transcriptional memory in post-mitotic cells.