Core Facilities - X-Ray Crystallography Laboratory

Sándor BROCKHAUSER
senior research associate

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Veronika HARMAT research associate
Katalin MATÓ council administrator
Valéria BUGRIS research associate

X-RAY CRYSTALLOGRAPHY LABORATORY

Molecule structure determination at atomic level explores the structural basis of their reactions, interactions and biological activity. The X-Ray Crystallography Laboratory equipped with a microfocus X-ray diffractometer and crystallization facilities serves for solving the strucutres of both small molecules and proteins.

Infrastructure:
Röntgen-diffraktométer:

  • - Bruker Microstar 2 port rotating anode microfocus generator (Cu K-alpha radiation), data collection from two samples simultaneously
  • - Kappa goniometer and Bruker Smart 6000 CCD detector equipped with és 2-theta arm
  • - MAR 345 image plate detector
  • - Oxford Cryostream 700 and Oxford Cryostream 600

Zeiss confocal microscopes
Crystallization lab.

The diffractometer facilitates data collection from two crystals independently and simultaneously. The MAR 345 detector is ideal for studying protein crystals with long unit cell axes, while using the Bruker Smart 6000 CCD detector atomic resolution dataset can be collected form smal molecular or protein crystals. Using the microfocus generator also small and wealky diffracting crystals can be screened or high resolution data sets can be collected from good quality crystals. Low temperature data collection can be carried out routinely using the cryostream system.

Profile:

  • - Crystallization of proteins
  • - Data collection from small molecule crystals (from room temperature to cryogenic temperatures)
  • - Fast screening of protein crystals fro preparing data collection at a synchrotron source
  • - Collection of high resolution datasets from good quality protein crystals
  • - Solving the structures

MAR 345 image plate detector

Bruker Smart 6000 CCD detector

Course:
2013-14 KDIT 92-1 X-ray crystallography