Plasmid and fragment DNAs have been sequenced using fluorescent dye-labelled (BigDye) terminators and cycle sequencing method in this laboratory for several years. From the beginning of 2003, the runs and the analyses of the sequencing reactions are done on an ABI Prism 3100 Genetic Analyzer capillary sequencer. Using the 50 cm capillaries, the obtained DNA sequences are reliable up to 600-700 nucleotides. Results can be transferred to a paper form (printed Electropherograms) or to an electronic platform.
This laboratory operates as a custom-service facility and charges a fee for DNA sequence determinations.